nonimmune rat serum Search Results


nonimmune rat serum  (Jackson Immuno)
93
Jackson Immuno nonimmune rat serum
Nonimmune Rat Serum, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonimmune+rat+serum/pmc02148200-30-11-14?v=Jackson+Immuno
Average 93 stars, based on 1 article reviews
nonimmune rat serum - by Bioz Stars, 2026-07
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rat nonimmune isotype control igg  (Valiant Co Ltd)
94
Valiant Co Ltd rat nonimmune isotype control igg
IL-6 blockade inhibits accumulation of Th17 cells in the murine lung after hypoxia exposure. (A) qRT-PCR analysis of Il-17A mRNA expression in the lungs of C57BL/6 WT mice after hypoxia exposure. The results are pooled data from at least three independent experiments with 5–10 mice per group. (B–E) qRT-PCR analysis of IL-17A (B), Rorc (C), Cxcl1 (D), and Cxcl5 (E) mRNA expression in the lungs of mice treated with control antibody <t>or</t> <t>MR16-1</t> after exposure to hypoxia or normoxia for 2 d (n = 8). (F and G) Western blot analysis of IL-17A in the lung homogenates from mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 2 d (n = 6). Relative levels of IL-17A protein (normalized to β-tubulin) compared with the normoxic control group are shown. (H) Flow cytometric analysis of IL-17A–expressing cells in the CD4+ gated T-cell population isolated from the lungs of mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 3 d (n = 6). (I) Percentage of IL-17A+ cells within the CD4+ population. Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. (J) Experimental protocol for examining the effect of anti–IL-17A antibody treatment on HPH. An anti–IL-17A neutralizing monoclonal antibody or control antibody <t>(IgG)</t> was administered to C57BL/6 mice under hypoxic conditions. (K and L) Assessment of antibody-treated mice. RVSP (n = 6) (K) and Fulton's index (n = 6) (L) are shown. (M–O) IL-17A blockade significantly attenuates the hypoxia-induced IL-21 up-regulation in the murine lungs. (M) qRT-PCR analysis of IL-21 mRNA expression in the lungs of mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). (N and O) Western blot analysis of IL-21 in the lung homogenates from mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. NS, not significant.
Rat Nonimmune Isotype Control Igg, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonimmune+rat+serum/pmc04443312-315-21-26?v=Valiant+Co+Ltd
Average 94 stars, based on 1 article reviews
rat nonimmune isotype control igg - by Bioz Stars, 2026-07
94/100 stars
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nonimmune iggs  (Vector Laboratories)
96
Vector Laboratories nonimmune iggs
IL-6 blockade inhibits accumulation of Th17 cells in the murine lung after hypoxia exposure. (A) qRT-PCR analysis of Il-17A mRNA expression in the lungs of C57BL/6 WT mice after hypoxia exposure. The results are pooled data from at least three independent experiments with 5–10 mice per group. (B–E) qRT-PCR analysis of IL-17A (B), Rorc (C), Cxcl1 (D), and Cxcl5 (E) mRNA expression in the lungs of mice treated with control antibody <t>or</t> <t>MR16-1</t> after exposure to hypoxia or normoxia for 2 d (n = 8). (F and G) Western blot analysis of IL-17A in the lung homogenates from mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 2 d (n = 6). Relative levels of IL-17A protein (normalized to β-tubulin) compared with the normoxic control group are shown. (H) Flow cytometric analysis of IL-17A–expressing cells in the CD4+ gated T-cell population isolated from the lungs of mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 3 d (n = 6). (I) Percentage of IL-17A+ cells within the CD4+ population. Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. (J) Experimental protocol for examining the effect of anti–IL-17A antibody treatment on HPH. An anti–IL-17A neutralizing monoclonal antibody or control antibody <t>(IgG)</t> was administered to C57BL/6 mice under hypoxic conditions. (K and L) Assessment of antibody-treated mice. RVSP (n = 6) (K) and Fulton's index (n = 6) (L) are shown. (M–O) IL-17A blockade significantly attenuates the hypoxia-induced IL-21 up-regulation in the murine lungs. (M) qRT-PCR analysis of IL-21 mRNA expression in the lungs of mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). (N and O) Western blot analysis of IL-21 in the lung homogenates from mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. NS, not significant.
Nonimmune Iggs, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonimmune+rat+serum/pmc03630993-79-2-14?v=Vector+Laboratories
Average 96 stars, based on 1 article reviews
nonimmune iggs - by Bioz Stars, 2026-07
96/100 stars
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igg  (SouthernBiotech)
96
SouthernBiotech igg
IL-6 blockade inhibits accumulation of Th17 cells in the murine lung after hypoxia exposure. (A) qRT-PCR analysis of Il-17A mRNA expression in the lungs of C57BL/6 WT mice after hypoxia exposure. The results are pooled data from at least three independent experiments with 5–10 mice per group. (B–E) qRT-PCR analysis of IL-17A (B), Rorc (C), Cxcl1 (D), and Cxcl5 (E) mRNA expression in the lungs of mice treated with control antibody <t>or</t> <t>MR16-1</t> after exposure to hypoxia or normoxia for 2 d (n = 8). (F and G) Western blot analysis of IL-17A in the lung homogenates from mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 2 d (n = 6). Relative levels of IL-17A protein (normalized to β-tubulin) compared with the normoxic control group are shown. (H) Flow cytometric analysis of IL-17A–expressing cells in the CD4+ gated T-cell population isolated from the lungs of mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 3 d (n = 6). (I) Percentage of IL-17A+ cells within the CD4+ population. Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. (J) Experimental protocol for examining the effect of anti–IL-17A antibody treatment on HPH. An anti–IL-17A neutralizing monoclonal antibody or control antibody <t>(IgG)</t> was administered to C57BL/6 mice under hypoxic conditions. (K and L) Assessment of antibody-treated mice. RVSP (n = 6) (K) and Fulton's index (n = 6) (L) are shown. (M–O) IL-17A blockade significantly attenuates the hypoxia-induced IL-21 up-regulation in the murine lungs. (M) qRT-PCR analysis of IL-21 mRNA expression in the lungs of mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). (N and O) Western blot analysis of IL-21 in the lung homogenates from mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. NS, not significant.
Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nonimmune+rat+serum/10__1164_slash_rccm__200410___1398oc-39-4-7?v=SouthernBiotech
Average 96 stars, based on 1 article reviews
igg - by Bioz Stars, 2026-07
96/100 stars
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Image Search Results


IL-6 blockade inhibits accumulation of Th17 cells in the murine lung after hypoxia exposure. (A) qRT-PCR analysis of Il-17A mRNA expression in the lungs of C57BL/6 WT mice after hypoxia exposure. The results are pooled data from at least three independent experiments with 5–10 mice per group. (B–E) qRT-PCR analysis of IL-17A (B), Rorc (C), Cxcl1 (D), and Cxcl5 (E) mRNA expression in the lungs of mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 2 d (n = 8). (F and G) Western blot analysis of IL-17A in the lung homogenates from mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 2 d (n = 6). Relative levels of IL-17A protein (normalized to β-tubulin) compared with the normoxic control group are shown. (H) Flow cytometric analysis of IL-17A–expressing cells in the CD4+ gated T-cell population isolated from the lungs of mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 3 d (n = 6). (I) Percentage of IL-17A+ cells within the CD4+ population. Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. (J) Experimental protocol for examining the effect of anti–IL-17A antibody treatment on HPH. An anti–IL-17A neutralizing monoclonal antibody or control antibody (IgG) was administered to C57BL/6 mice under hypoxic conditions. (K and L) Assessment of antibody-treated mice. RVSP (n = 6) (K) and Fulton's index (n = 6) (L) are shown. (M–O) IL-17A blockade significantly attenuates the hypoxia-induced IL-21 up-regulation in the murine lungs. (M) qRT-PCR analysis of IL-21 mRNA expression in the lungs of mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). (N and O) Western blot analysis of IL-21 in the lung homogenates from mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. NS, not significant.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Interleukin-6/interleukin-21 signaling axis is critical in the pathogenesis of pulmonary arterial hypertension

doi: 10.1073/pnas.1424774112

Figure Lengend Snippet: IL-6 blockade inhibits accumulation of Th17 cells in the murine lung after hypoxia exposure. (A) qRT-PCR analysis of Il-17A mRNA expression in the lungs of C57BL/6 WT mice after hypoxia exposure. The results are pooled data from at least three independent experiments with 5–10 mice per group. (B–E) qRT-PCR analysis of IL-17A (B), Rorc (C), Cxcl1 (D), and Cxcl5 (E) mRNA expression in the lungs of mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 2 d (n = 8). (F and G) Western blot analysis of IL-17A in the lung homogenates from mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 2 d (n = 6). Relative levels of IL-17A protein (normalized to β-tubulin) compared with the normoxic control group are shown. (H) Flow cytometric analysis of IL-17A–expressing cells in the CD4+ gated T-cell population isolated from the lungs of mice treated with control antibody or MR16-1 after exposure to hypoxia or normoxia for 3 d (n = 6). (I) Percentage of IL-17A+ cells within the CD4+ population. Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. (J) Experimental protocol for examining the effect of anti–IL-17A antibody treatment on HPH. An anti–IL-17A neutralizing monoclonal antibody or control antibody (IgG) was administered to C57BL/6 mice under hypoxic conditions. (K and L) Assessment of antibody-treated mice. RVSP (n = 6) (K) and Fulton's index (n = 6) (L) are shown. (M–O) IL-17A blockade significantly attenuates the hypoxia-induced IL-21 up-regulation in the murine lungs. (M) qRT-PCR analysis of IL-21 mRNA expression in the lungs of mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). (N and O) Western blot analysis of IL-21 in the lung homogenates from mice treated with control antibody or an anti–IL-17A neutralizing antibody after exposure to hypoxia or normoxia for 2 d (n = 3). Values shown are the mean ± SEM; *P < 0.05, **P < 0.01 calculated using ANOVA. NS, not significant.

Article Snippet: To determine the effect of the MR16-1–mediated blockade of IL-6, mice were i.v. injected with 2 mg of MR16-1 or purified rat nonimmune isotype control IgG (MP Biomedicals) just before exposure to hypoxia or normoxia and subsequently were injected i.p. with 0.5 mg of MR16-1 or control IgG, respectively, once a week.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Isolation